ClickChemistryTools產(chǎn)品27-Alkyne cholesterol英文介紹

27-Alkyne cholesterol is a modified lipid containing an omega-terminal alkyne. The terminal alkyne group can be used in a highly specific linking reaction with azide-containing reagents, known as ‘click chemistry’, in the presence of a copper-containing catalyst.

Alkyne cholesterol represents a versatile, sensitive, and easy-to-use tool for tracking cellular cholesterol metabolism and localization as it allows for manifold detection methods including mass spectrometry, and fluorescence microscopy.

27-Alkyne cholesterol is accepted by cellular enzymes from different biological species (Brevibacterium, yeast, rat, human) and these enzymes include cholesterol oxidases, hydroxylases, and acyl transferases that generate the expected metabolites in in vitro and in vivo assays. Using fluorescence microscopy, researchers can study the distribution of cholesterol at subcellular resolution, detecting the lipid in the Golgi and at the plasma membrane, but also in the endoplasmic reticulum and mitochondria.

In summary, alkyne cholesterol represents a versatile, sensitive, and easy-to-use tool for tracking cellular cholesterol metabolism and localization as it allows for manifold detection methods including mass spectrometry, and fluorescence microscopy.

ClickChemistryTools產(chǎn)品27-Alkyne cholesterol中文介紹

27-炔烴膽固醇是一種含有歐米茄末端炔烴的改性脂質(zhì)。在含銅催化劑存在下，末端炔烴基可用于與含疊氮化物試劑的高度特異性

連接反應(yīng)，稱(chēng)為“點(diǎn)擊化學(xué)"。

炔烴膽固醇是一種多功能、靈敏且易于使用的工具，用于跟蹤細(xì)胞膽固醇代謝和定位，因?yàn)樗试S多種檢測(cè)方法，包括質(zhì)譜和熒光

顯微鏡。

27-炔烴膽固醇被來(lái)自不同生物物種（短桿菌、酵母、大鼠、人）的細(xì)胞酶所接受，這些酶包括膽固醇氧化酶、羥化酶和酰基轉(zhuǎn)移

酶，它們?cè)隗w外和體內(nèi)測(cè)定中產(chǎn)生預(yù)期的代謝物。使用熒光顯微鏡，研究人員可以在亞細(xì)胞分辨率下研究膽固醇的分布，檢測(cè)高爾

基體和質(zhì)膜中的脂質(zhì)，還可以檢測(cè)內(nèi)質(zhì)網(wǎng)和線(xiàn)粒體中的脂質(zhì)。

總之，炔烴膽固醇是一種多功能、靈敏且易于使用的工具，用于跟蹤細(xì)胞膽固醇代謝和定位，因?yàn)樗试S多種檢測(cè)方法，包括質(zhì)譜

和熒光顯微鏡。

Selected References

1.Friedrich, F., et al. (2021). Direct Measurement of Intermolecular Mechanical Force for Nonspecific Interactions between Small Molecules. J Phys Chem Lett., 12 (46), 11316-11322.

2.Hofmann K., et al. (2014). A novel alkyne cholesterol to trace cellular cholesterol metabolism and localization. J. Lipid. Res., 55, 583-91.